NEDD4 抗体 (Internal Region)
-
- 抗原 See all NEDD4 抗体
- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
-
抗原表位
- Internal Region
-
适用
- 人, 马, Macaque, 兔, 犬, Chimpanzee
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This NEDD4 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody reacts with endogenous Nedd4 protein.
- 交叉反应 （详细）
-
Species reactivity (expected):Horse, Macaque, Chimpanzee, Rabbit, Canine, Cattle.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 免疫原
- Peptide corresponding to an internal portion of the Nedd4 protein
- 亚型
- IgG
- Top Product
- Discover our top product NEDD4 Primary Antibody
-
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319) antibody
NEDD4 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 160-460) antibodyNEDD4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 801-900) antibodyNEDD4 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibodyNEDD4 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 1288-1319), (C-Term) antibodyNEDD4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15462 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) antibodyNEDD4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 950-1000) antibodyNEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 200-250) antibodyNEDD4 适用: 人, 大鼠, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (AA 324-373) antibodyNEDD4 适用: 人, 马, 猴, 兔, 犬, Cow, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4) (Internal Region) antibodyNEDD4 适用: 人, 马, Macaque WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
ELISA: 1/20,000 - 1/35,000. Western Blot: 1/300 to 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 0.64 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
-
- 抗原
- NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))
- 别名
- NEDD4 (NEDD4 产品)
- 别名
- NEDD4-1 antibody, RPF1 antibody, AA959633 antibody, AL023035 antibody, AU019897 antibody, E430025J12Rik antibody, Nedd4-1 antibody, Nedd4a antibody, mKIAA0093 antibody, CG32184 antibody, CG42279 antibody, CG7555 antibody, DNedd4 antibody, Dmel\\CG42279 antibody, Dmel_CG32184 antibody, Dmel_CG7555 antibody, dNedd4 antibody, dNedd4-1 antibody, nedd4 antibody, GB16853 antibody, NEDD4 antibody, Nedd4 antibody, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody, neural precursor cell expressed, developmentally down-regulated 4 antibody, CG42279 gene product from transcript CG42279-RJ antibody, E3 ubiquitin-protein ligase Nedd-4 antibody, E3 ubiquitin-protein ligase NEDD4 antibody, NEDD4 antibody, Nedd4 antibody, LOC411723 antibody, LOC100457150 antibody, nedd4 antibody, LOC100547485 antibody
- 背景
- NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.Synonyms: E3 ubiquitin-protein ligase NEDD4, KIAA0093
- 基因ID
- 4734
- NCBI登录号
- NP_006145
- UniProt
- P46934
- 途径
- Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2
-
