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EGFR 抗体 (Tyr1173)

Name: EGFR 抗体 (Tyr1173)
Brand: antibodies-online
SKU: ABIN343717
Availability: OutOfStock

EGFR 适用: 人 WB, FACS, IP 宿主: 小鼠 Monoclonal EM-13 unconjugated

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 inEGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions). (EGFR 抗体 (Tyr1173))

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 μg/mL, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining). (EGFR 抗体 (Tyr1173))

FACS

Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody (C)and anti-EGFR monoclonal mAb108 (D). (EGFR 抗体 (Tyr1173))

Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody(C) and anti-EGFR monoclonal mAb108 (D). (EGFR 抗体 (Tyr1173))

4 images

产品编号 ABIN343717

发货至: 中国

Datasheet as PDF

抗原 See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))
抗原表位
41

39

39

38

35

28

27

27

22

21

19

19

18

17

16

16

15

15

15

14

14

13

13

11

9

8

7

7

7

6

5

5

5

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2
Tyr1173
适用
805

327

317

26

26

24

20

15

9

3

3

2

1

1

1

1

1

1

1
人
宿主
635

173

14

11

5

1
小鼠
Compatible Secondaries
克隆类型
583

251

2
单克隆
标记
427

63

32

30

29

29

29

27

27

25

23

15

10

10

10

10

10

5

4

4

4

4

4

1

1

1

1

1

1

1

1
This EGFR antibody is un-conjugated
应用范围
563

262

146

143

137

101

100

96

81

55

37

34

34

18

10

8

6

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

特异性

The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

克隆位点

EM-13

亚型

IgG1

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anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
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应用备注

Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR (EGFR 产品)

别名

C-erb antibody, CG10079 antibody, D-EGFR antibody, D-Egf antibody, DEGFR antibody, DER antibody, DER flb antibody, DER/EGFR antibody, DER/faint little ball antibody, DER/top antibody, DER/torpedo antibody, DER1 antibody, DEgfr antibody, Degfr antibody, Der antibody, DmHD-33 antibody, Dmel\\CG10079 antibody, EFG-R antibody, EGF-R antibody, EGFR antibody, EGFr antibody, EGfr antibody, EK2-6 antibody, Egf antibody, Egf-r antibody, EgfR antibody, El antibody, Elp antibody, Elp-1 antibody, Elp-B1 antibody, Elp-B1RB1 antibody, HD-33 antibody, TOP antibody, Torpedo/DER antibody, Torpedo/Egfr antibody, c-erbB antibody, d-egf-r antibody, dEGFR antibody, dEGFR1 antibody, dEgfr antibody, der antibody, egfr antibody, flb antibody, l(2)05351 antibody, l(2)09261 antibody, l(2)57DEFa antibody, l(2)57EFa antibody, l(2)57Ea antibody, mor1 antibody, top antibody, top/DER antibody, top/flb antibody, torpedo/Egfr antibody, torpedo/egfr antibody, EGFR12 antibody, EGFR15 antibody, egfr1 antibody, Erbb2 antibody, ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, ErbB-1 antibody, Errp antibody, 9030024J15Rik antibody, AI552599 antibody, Erbb antibody, Errb1 antibody, Wa5 antibody, wa-2 antibody, wa2 antibody, epidermal growth factor receptor antibody, Epidermal growth factor receptor antibody, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antibody, EGFR antibody, Egfr antibody, egfra antibody, egfr1 antibody, LOC5564544 antibody

背景

Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

基因ID

1956

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins