TRIM28 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRIM28 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of TRIM28.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 100-120 kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/mL (Predicted MW Approximately 92 kDa according to NP_005753). Immunohistochemistry: This antibody has been successfully used on human gastric tumor surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5 μg/mL. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.