LEF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
LEF1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of LEF1.
ELISA: Antibody detection limit dilution 1:32,000. Approximately 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al, EMBO J. 2002 Jun 3,21 (11) :2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.