Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
NFκB-p100/p52 antibody detects endogenous levels of total NFκB-p100/p52 protein.
纯化方法
Affinity chromatography
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human NFkappaB-p100/p52 around the phosphorylation site of serine 871 (S-Q-SP-V-E).
NFKB2
适用: 人, 小鼠
ELISA, IHC (p), ICC, FACS, IF (p), IF (cc), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
抗原
NFKB2
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))
NF-kappaB2 antibody, lyt antibody, p49 antibody, p49/p100 antibody, p50B antibody, p52 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100 antibody, Nfkb2 antibody
背景
NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.Synonyms: DNA-binding factor KBF2, H2TF1, KBF2, LYT10, Lymphocyte translocation chromosome 10, NF kappa B, NF-kB p100, NF-kB p52, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10