NFKB2 抗体 (pSer866)
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北京 101111
Quick Overview for NFKB2 抗体 (pSer866) (ABIN6256439)
抗原
See all NFKB2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer866
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特异性
- Phospho-NF kappaB p100/p52 (Ser866) Antibody detects endogenous levels of NF kappaB p100/p52 only when phosphorylated at Serine 866.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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免疫原
- A synthesized peptide derived from human NF- kappaB p100/p52 around the phosphorylation site of Ser866.
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亚型
- IgG
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anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 690-899) antibody
NFKB2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 1-340) antibodyNFKB2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (C-Term) antibodyNFKB2 适用: 人, 大鼠, Cow, Pig, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (pSer870) antibodyNFKB2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (C-Term) antibodyNFKB2 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 712-900) antibodyNFKB2 适用: 人, 小鼠 ELISA 宿主: 小鼠 Monoclonal 5E6A12 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 712-900) antibodyNFKB2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2F10A7 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 151-250) antibodyNFKB2 适用: 人, 小鼠 ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (AA 38-343) antibodyNFKB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2) (pSer866) antibodyNFKB2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- NFKB2 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))
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别名
- NFKB2
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背景
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Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form, the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
Gene: NFKB2
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分子量
- 100kDa
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基因ID
- 4791
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UniProt
- Q00653
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途径
- Toll-Like Receptors Cascades
抗原
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