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CHEK2 抗体 (N-Term)

Name: CHEK2 抗体 (N-Term)
SKU: ABIN2840120
Availability: OutOfStock

CHEK2 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB19067 unconjugated

CHEK2 Antibody (N-term) (ABIN1881196 and ABIN2840120) western blot analysis in K562,NCI- cell line lysates (35 μg/lane). (CHEK2 抗体 (N-Term))

CHEK2 Antibody (N-term) (ABIN1881196 and ABIN2840120) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (CHEK2 抗体 (N-Term))

FACS

Confocal immunofluorescent analysis of CHEK2 Antibody (N-term) (ABIN1881196 and ABIN2840120) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (CHEK2 抗体 (N-Term))

All lanes : Anti-CHEK2 Antibody (N-term) at 1:1000 dilution Lane 1: HC whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. (CHEK2 抗体 (N-Term))

CHEK2 Antibody (N-term) (ABIN1881196 and ABIN2840120) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (CHEK2 抗体 (N-Term))

IHC (p)

5 images

(5 references)

产品编号 ABIN2840120

发货至: 中国

Datasheet as PDF

抗原 See all CHEK2 抗体

CHEK2 (Checkpoint Kinase 2 (CHEK2))
抗原表位
30

28

21

17

16

15

15

15

9

8

8

8

8

7

7

6

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 111-141, N-Term
适用
261

96

82

16

14

7

6

5

2

2

2

1

1
人
宿主
242

20

1

1
兔
Compatible Secondaries
克隆类型
228

36
多克隆
标记
116

14

12

12

12

12

12

12

10

9

7

7

7

7

7

2

2

2

1

1
This CHEK2 antibody is un-conjugated
应用范围
206

80

79

72

37

36

33

28

28

19

18

5

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.

克隆位点

RB19067

亚型

Ig Fraction

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CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibody
CHEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibody
CHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pThr383) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibody
CHEK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Xiao, Zheng, Huang, Liu, Zhang, Ma: "Deficiency of 53BP1 inhibits the radiosensitivity of colorectal cancer." in: International journal of oncology, Vol. 49, Issue 4, pp. 1600-8, (2017) (PubMed).

Yao, Huang, Zheng, Liu, Lin, Zhang, Yang, Zhang, Ma: "53BP1 loss induces chemoresistance of colorectal cancer cells to 5-fluorouracil by inhibiting the ATM-CHK2-P53 pathway." in: Journal of cancer research and clinical oncology, Vol. 143, Issue 3, pp. 419-431, (2017) (PubMed).

Yang, Wood, Hrushesky: "Mammalian TIMELESS is required for ATM-dependent CHK2 activation and G2/M checkpoint control." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3030-4, (2010) (PubMed).

Varmark, Kwak, Theurkauf: "A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 312-20, (2010) (PubMed).

Zhu, Miao, Huang, Feng, Zhang, Lu, Cai, Tong, Xu, Qian, Ding: "Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 11, pp. 1226-34, (2009) (PubMed).
抗原

CHEK2 (Checkpoint Kinase 2 (CHEK2))

别名

CHEK2 (CHEK2 产品)

别名

CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody

背景

CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53.

分子量

60915

NCBI登录号

NP_001005735, NP_001244316, NP_009125, NP_665861

UniProt

O96017

途径

p53 Pathway, Apoptosis, Cell Division Cycle