我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

GFP 抗体

Name: GFP 抗体
SKU: ABIN1842274
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

(3 references)

产品编号 ABIN1842274

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
73

73

35

11

9

6

2

1
兔
Compatible Secondaries
克隆类型
120

85
多克隆
标记
103

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
172

87

54

42

33

30

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

品牌

THE™

特异性

THETM GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.

产品特性

THE™ GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.

纯化方法

Immunoaffinity chromatography

免疫原

purified recombinant full-length GFP protein

亚型

IgG

Top Product

Discover our top product GFP Primary Antibody
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0. 2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
Flow cytometry: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

浓度

0.5 mg/mL

缓冲液

lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Krager, Sarkar, Twait, Lill, Koland: "A novel biotinylated lipid raft reporter for electron microscopic imaging of plasma membrane microdomains." in: Journal of lipid research, Vol. 53, Issue 10, pp. 2214-25, (2012) (PubMed).

Kadauke, Udugama, Pawlicki, Achtman, Jain, Cheng, Hardison, Blobel: "Tissue-specific mitotic bookmarking by hematopoietic transcription factor GATA1." in: Cell, Vol. 150, Issue 4, pp. 725-37, (2012) (PubMed).

Du, Jaggi, Zhang, Balaji: "Protein kinase D1-mediated phosphorylation and subcellular localization of beta-catenin." in: Cancer research, Vol. 69, Issue 3, pp. 1117-24, (2009) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y