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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN1574240
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, IP 宿主: 小鸡 Polyclonal unconjugated

(1 reference)

产品编号 ABIN1574240

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

35

10

9

6

2

1
小鸡
克隆类型
120

85
多克隆
标记
103

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
172

87

55

42

32

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP)

特异性

GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.

交叉反应（详细）

GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.

纯化方法

Immunoaffinity chromatography

免疫原

purified recombinant full-length GFP protein

亚型

IgG2a

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应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Immunoprecipitation: 1-5 µg/mL Other Applications: user optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
McIntyre, Davis, Joiner, Williams, Tsai, Jenkins, McEwen, Zhang, Escobado, Thomas, Szymanska, Johnson, Beales, Green, Mullikin, Sabo, Muzny, Gibbs, Attié-Bitach, Yoder, Reed, Katsanis, Martens: "Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy model." in: Nature medicine, Vol. 18, Issue 9, pp. 1423-8, (2012) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. GFP Antibody, pAb, Chicken is developed in chicken using purified recombinant full-length GFP protein.