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E-cadherin 抗体 (AA 650-700)

Name: E-cadherin 抗体 (AA 650-700)
Brand: antibodies-online
SKU: ABIN1574190
Availability: OutOfStock

CDH1 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated

Western blot analysis of cell lysates using E-Cadherin Antibody (ABIN399088, 1 µg/mL) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. (E-cadherin 抗体 (AA 650-700))

Flow cytometric analysis of HEK293 cells using E-Cadherin Antibody (ABIN399088, shaded histogram) or with an isotype control antibody (ABIN398653, open histogram), followed by R-PE conjugated anti-rabbit IgG. (E-cadherin 抗体 (AA 650-700))

FACS

2 images

(3 references)

产品编号 ABIN1574190

发货至: 中国

Datasheet as PDF

抗原 See all E-cadherin (CDH1) 抗体

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
抗原表位
17

17

16

11

10

9

8

8

7

7

5

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 650-700
适用
222

94

82

18

17

12

9

9

9

6

4

4

4

4

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
190

70

7

3
兔
Compatible Secondaries
克隆类型
178

91

1
多克隆
标记
130

34

28

8

7

6

6

6

6

6

6

6

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This E-cadherin antibody is un-conjugated
应用范围
189

88

80

63

51

49

36

33

29

27

17

16

5

5

4

4

3

3

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS)

特异性

E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology.Positive Control: HepG2 and NIH/3T3

交叉反应（详细）

E-Cadherin Antibody detects human and mouse endogenous levels of E-Cadherin. It is predicted to react with rat E-Cadherin protein according to sequence homology.Positive Control: HepG2 and NIH/3T3

纯化方法

Immunoaffinity chromatography

免疫原

KLH-coupled synthetic peptide within AA 650–700 of human E-Cadherin

亚型

IgG

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anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 犬, 小鸡, 兔, Cow, 马, 豚鼠, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IHC 宿主: 山羊 Polyclonal unconjugated

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应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Dong, Kaneuchi, Xiong, Cao, Cai, Liu, Guo, Ju, Jia, Konno, Watari, Hosaka, Sudo, Sakuragi: "Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer." in: Carcinogenesis, Vol. 35, Issue 4, pp. 760-8, (2014) (PubMed).

Konno, Dong, Xiong, Suzuki, Lu, Cai, Watari, Mitamura, Hosaka, Hanley, Kudo, Sakuragi: "MicroRNA-101 targets EZH2, MCL-1 and FOS to suppress proliferation, invasion and stem cell-like phenotype of aggressive endometrial cancer cells." in: Oncotarget, Vol. 5, Issue 15, pp. 6049-62, (2014) (PubMed).

Erin, Kale, Tanr?över, Köksoy, Duymu?, Korcum: "Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma." in: Breast cancer research and treatment, Vol. 139, Issue 3, pp. 677-89, (2013) (PubMed).
抗原

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

别名

E-Cadherin (CDH1 产品)

别名

Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

背景

E-Cadherin is a key cell adhesion molecule located at adherens junctions (AJs) and undergoes endocytosis when AJs are disrupted by the action of extracellular signals. It is a member of cadherin family that includes cadhesin repeats of approximately 100 residues in their extracellular domian. E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is an important molecule in maintaining epithelial integrity. The expression of E-Cadherin might be associated with the invasive potential of epithelial neoplasms. Defects in E-Cadherin are a cause of gastric cancer, also known as hereditary familial diffuse gastric cancer (HDGC).E-Cadherin Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 650-00 of human E-Cadherin (Swiss Prot: P12830).

途径

WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation