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WNT1 抗体

WNT1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN155191
发货至: 中国
  • 抗原 See all WNT1 抗体
    WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This WNT1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    特异性
    The antibody recognizes Human WNT-1. Other species not tested.
    纯化方法
    Affinity chromatography employing immobilized hWNT-1 matrix
    免疫原
    Highly pure (>98%) recombinant human WNT-1
    Top Product
    Discover our top product WNT1 Primary Antibody
  • 应用备注
    ElisaIndirect: To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hWNT-1. Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western BlotTo detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    有效期
    12 months
  • 抗原
    WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))
    别名
    WNT1 (WNT1 产品)
    别名
    Xint-1 antibody, Xwnt1 antibody, int-1 antibody, int1 antibody, wnt-1 antibody, wnt1-a antibody, Int-1 antibody, Wnt-1 antibody, sw antibody, swaying antibody, BMND16 antibody, INT1 antibody, OI15 antibody, Int1 antibody, WNT-1 antibody, sb:eu647 antibody, zgc:194464 antibody, zgc:194478 antibody, WNT1 antibody, Wnt family member 1 antibody, Wnt family member 1 L homeolog antibody, wingless-type MMTV integration site family, member 1 antibody, WNT1 antibody, wnt1.L antibody, Wnt1 antibody, wnt1 antibody
    背景
    Synonyms: INT-1, INT1, Proto-oncogene protein Wnt-1
    基因ID
    7471
    NCBI登录号
    NP_005421
    UniProt
    P04628
    途径
    WNT signaling, Dopaminergic Neurogenesis
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