ElisaIndirect: To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hWNT-1. Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western BlotTo detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
有效期
12 months
抗原
WNT1
(Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))