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CHEK2 抗体 (pThr68)

Name: CHEK2 抗体 (pThr68)
Brand: antibodies-online
SKU: ABIN129576
Availability: OutOfStock

CHEK2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. (CHEK2 抗体 (pThr68))

1 image

(1 reference)

产品编号 ABIN129576

发货至: 中国

Datasheet as PDF

抗原 See all CHEK2 抗体

CHEK2 (Checkpoint Kinase 2 (CHEK2))
抗原表位
28

24

21

17

16

15

15

15

9

8

8

8

8

7

7

6

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 64-73, pThr68
适用
254

96

82

16

14

7

6

5

2

2

2

1

1
人
宿主
235

20

1

1
兔
Compatible Secondaries
克隆类型
227

30
多克隆
标记
113

14

12

12

12

12

12

12

9

9

7

7

7

7

7

2

2

1
This CHEK2 antibody is un-conjugated
应用范围
203

80

79

71

37

35

32

27

23

19

17

5

1

1

1

1

1
Western Blotting (WB), ELISA

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 64-73 of Human CHK2.

亚型

IgG

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CHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated

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anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody
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anti-Checkpoint Kinase 2 (CHEK2) (pThr383) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibody
CHEK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appropriate cell lysate or extract. Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pT68 of CHK2.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Lu, Lou, Peng, Xie, Nghiem, Conney: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." in: Cancer research, Vol. 68, Issue 7, pp. 2523-9, (2008) (PubMed).
抗原

CHEK2 (Checkpoint Kinase 2 (CHEK2))

别名

CHK2 (CHEK2 产品)

别名

CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody

背景

CHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/ threonine-protein kinase involved in the control of cell cycle checkpoints and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.
Synonyms: Serine/threonine-protein kinase Chk2 EC=2.7.11.1 Cds1

基因ID

11200

NCBI登录号

NP_001005735

UniProt

O96017

途径

p53 Pathway, Apoptosis, Cell Division Cycle