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- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
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宿主
- 小鼠
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克隆类型
- 多克隆
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标记
- This Phosphotyrosine antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 纯化方法
- Purified
- 组件
- incl. positive Control
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
- Positive Control for Immunonlot Applications. Phosphotyrosine Molecular Weight Marker:
- 限制
- 仅限研究用
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains. Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.
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