Phosphotyrosine 抗体
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- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphotyrosine antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Phosphotyrosine conjugated to KLH
- 克隆位点
- PY20
- 亚型
- IgG2b kappa
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
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- 应用备注
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Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Phosphotyrosine
- 别名
- Phosphotyrosine (P-Tyr) (Phosphotyrosine 产品)
- 物质类
- Amino Acid
- 背景
- Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
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