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Phosphotyrosine 抗体

Name: Phosphotyrosine 抗体
SKU: ABIN5775098
Availability: OutOfStock

WB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated

Western blot analysis of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates. (Phosphotyrosine 抗体)

ELISA

Immunofluorescent staining of EGF-stimulated A431 cells and untreated A431 cells with Phosphotyrosine monoclonal antibody, clone E10 . (Phosphotyrosine 抗体)

Flow cytometric analysis of EGF-stimulated A431 cells (red) and untreated A431 cells (black) with Phosphotyrosine monoclonal antibody, clone E10 . (Phosphotyrosine 抗体)

FACS

Western blot analysis of tyrosine phosphorylation status in different cells with Phosphotyrosine monoclonal antibody, clone E10 . (Phosphotyrosine 抗体)

5 images

产品编号 ABIN5775098

发货至: 中国

Datasheet as PDF

抗原 See all Phosphotyrosine products

Phosphotyrosine

适用

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宿主
88

24

1
小鼠
Compatible Secondaries
克隆类型
100

13
单克隆
标记
41

16

10

7

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
应用范围
79

55

35

33

31

22

22

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

原理

Mouse monoclonal antibody raised against Phosphotyrosine.

特异性

The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.

交叉反应（详细）

The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.

免疫原

Phosphotyrosine containing carrier protein.

克隆位点

E10

亚型

IgG1, IgG2a
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC

应用备注

ELISA (0.1 μg/mL)
Flow Cytometry (4 μg/mL)
Immunocytochemistry (4 μg/mL)
Immunofluorescence (4 μg/mL)
Immunoprecipitation (1-4 μg/mL)
Western Blot (1-2 μg/mL)
The optimal working dilution should be determined by the end user.

限制

仅限研究用
状态

Lyophilized

缓冲液

Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02 % sodium azide).

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C or lower.
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
抗原

Phosphotyrosine

Abstract

Phosphotyrosine 产品

物质类

Amino Acid