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Phosphotyrosine 抗体

Name: Phosphotyrosine 抗体
SKU: ABIN5774320
Availability: OutOfStock

WB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated

Western blot analysis of serum-starved A431 cells nontreated or treated with 100 ng/mL EGF using Phosphotyrosine monoclonal antibody, clone RM111 at 1:5000 dilution. (Phosphotyrosine 抗体)

Immunocytochemistry staining of serum-starved A431 cells (A) and serum-starved A431 cells treated with EGF (B) using Phosphotyrosine monoclonal antibody, clone RM111 (Red) at 1:500 dilution. (Phosphotyrosine 抗体)

ICC

Immunoprecipitation analysis of Lane 1: EGE-treated A431 cell whole lysates, Lane 2: EGE-treated A431 cell whole lysates using rabbit IgG antibody; Lane 3: EGE-treated A431 cell whole lysates using Phosphotyrosine monoclonal antibody, clone RM111 at 1:1000 dilution. (Phosphotyrosine 抗体)

3 images

产品编号 ABIN5774320

发货至: 中国

Datasheet as PDF

抗原 See all Phosphotyrosine products

Phosphotyrosine

适用

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宿主
89

23

1
兔
Compatible Secondaries
克隆类型
100

13
单克隆
标记
41

16

10

7

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
应用范围
79

55

35

32

32

22

22

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

原理

Recombinant rabbit monoclonal antibody raised against phosphotyrosine.

特异性

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine

交叉反应（详细）

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine

免疫原

Original antibody is raised against phosphotyrosine conjugated with BSA.

克隆位点

RM111

亚型

IgG
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-Phosphotyrosine antibody
适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC

应用备注

ELISA
Flow Cytometry
Immunocytochemistry (1:200-1:500)
Immunohistochemistry (1:200-1:500)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.

限制

仅限研究用
状态

Liquid

缓冲液

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
抗原

Phosphotyrosine

Abstract

Phosphotyrosine 产品

物质类

Amino Acid