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- 抗原 See all E-cadherin (CDH1) 抗体
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This E-cadherin antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody specifically recognizes E-cadherin at 124 kDa.
- 产品特性
- Synonyms: Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
- 纯化方法
- The antibody was purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.
- 组件
- incl. positive Control
- 免疫原
- Peptide conjugated to hemocyanin. Epitope: cytoplasmic domain.
- 克隆位点
- 22F8
- 亚型
- IgG1
- Top Product
- Discover our top product CDH1 Primary Antibody
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anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody
CDH1 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyKO Validated CDH1 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 381-480) antibodyCDH1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 3F4 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (C-Term) antibodyCDH1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 810-890) antibodyCDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 犬, 小鸡, 兔, Cow, 马, 豚鼠, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人 WB, IHC, IF, StM, ISt 宿主: 小鼠 Monoclonal CDH1-1525 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibodyCDH1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 155-707) antibodyCDH1 适用: 人 WB, FACS, ELISA, IF 宿主: 小鼠 Monoclonal 9H2D3 unconjugated
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- 应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated A431 cells as positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive control provided: Control cell lysate A 431 untreatedFormat: Lyophilized cell lysate from serum starved A431 cells. Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 2 x PBS / 0.09 % sodium azide / PEG and sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 4 °C up to 3 months. Avoid repeated freezing and thawing.
- 有效期
- 12 months
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- 抗原
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- 别名
- CD324 / Cadherin-1 (CDH1 产品)
- 别名
- Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody
- 背景
- Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed in most epithelial tissues.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin
- 基因ID
- 999
- UniProt
- P12830
- 途径
- WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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