(5 references)-
- 抗原 See all Collagen IV (COL4) 抗体
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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适用
- 人, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen IV antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- Collagen Type IV from human and bovine placenta.
- Top Product
- Discover our top product COL4 Primary Antibody
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 1043 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Suitable for indirect trapping ELISA for quantitation of antigen in serum using a standardcurve, for immunoprecipitation and for western blotting for highly sensitive qualitativeanalysis. Recommended Dilution(s): This product was assayed by immunoblot and found to bereactive against Collagen IV at a dilution of 1: 5,000 to 1: 10,000. This product was alsoassayed against 1.0 μg of Collagen IV in a standard sandwich ELISA using PeroxidaseConjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 4,000 to1: 8,000 of the stock concentration is suggested for this product. For Immunohistochemistry on frozen tissue sections dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 10 mg/mL BSA (Ig and Protease Free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
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: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).
: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).
: "Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses." in: Journal of leukocyte biology, Vol. 85, Issue 6, pp. 978-86, (2009) (PubMed).
: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).
: "Induction of neuregulin signaling in mouse schwann cells in vivo mimics responses to denervation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 25, pp. 6873-84, (2006) (PubMed).
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: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- Collagen Type IV (COL4 产品)
- 别名
- collagen, type IV antibody, Let-2 antibody
- 背景
- Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1
- 基因ID
- 9606
- UniProt
- P02462
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