GFP 抗体 (Green Fluorescent Protein) (AA 1-246)

Details for Product anti-GFP Antibody No. ABIN1043927, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
抗原表位
AA 1-246
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31
28
18
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1
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适用
Aequorea victoria
632
19
5
5
2
2
1
1
宿主
小鸡
335
172
81
42
16
9
9
2
克隆类型
多克隆
标记
This GFP antibody is un-conjugated
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30
28
14
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1
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1
应用范围
ELISA, Western Blotting (WB)
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25
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3
3
2
2
2
1
1
1
1
选项
Supplier
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免疫原 The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein
亚型 IgG
特异性 Anti-GFP Antibody was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).  No reaction was observed against Human, Mouse or Rat serum proteins.
产品特性 Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic
过滤 Sterile filtered
别名 GFP
背景 Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
应用备注 Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluor-escence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.
限制 仅限研究用
状态 Liquid
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
有效期 12 months
厂商提供的图像
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) Immunofluorescence Microscopy of Chicken anti-GFP antibody. Tissue: KruppleGAL4 drive...
 image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (Image 2)
 image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (Image 3)
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) Immunofluorescence Microscopy of Chicken anti-GFP antibody. Tissue: KruppleGAL4 drive...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) Western Blot of Anti-GFP (CHICKEN) antibody. Lane 1: MW. Lane 2: GFP. Load: 0.05 µg. ...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-246) antibody (ABIN1043927) Western Blot of Anti-GFP antibody. Lane 1: mouse spleen lysate. Lane 2: mouse spleen ...
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