anti-人 PKC gamma 抗体 for Enzyme Immunoassay

Recommended PKC gamma Antibody (supplied by: Log in to see )

抗原
Protein Kinase C, gamma (PRKCG) 抗体
  • PRKCG
  • PKC-gamma
  • PKCC
  • PKCG
  • SCA14
  • PKCgamma
  • Pkcc
  • Prkcc
  • PKC
  • PKCI
  • Prkc
  • RATPKCI
  • protein kinase C gamma
  • protein kinase C, gamma
  • protein kinase C gamma type
  • PRKCG
  • Prkcg
  • LOC484316
抗原表位
C-Term
33
18
16
15
15
14
14
7
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
147
145
127
7
7
6
4
4
4
3
2
2
2
1
宿主

194
5
1
克隆类型
多克隆
标记
This PKC gamma antibody is un-conjugated
12
10
8
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
153
71
39
33
23
12
6
3
2
2
1
1
1
1
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产品编号 ABIN359066
$ 390.50
加上运输费 $45.00

General

抗原 Protein Kinase C, gamma (PRKCG) 抗体
抗原表位 C-Term
(33), (18), (16), (15), (15), (14), (14), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(147), (145), (127), (7), (7), (6), (4), (4), (4), (3), (2), (2), (2), (1)
宿主
(194), (5), (1)
克隆类型
多克隆
标记 This PKC gamma antibody is un-conjugated
(12), (10), (8), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Enzyme Immunoassay (EIA), Western Blotting (WB)
(153), (71), (39), (33), (23), (12), (6), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5个引用可参考
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PKC gamma抗体详情

PKC gamma目标详情 使用细节 贮存及处理 References for anti-PKC gamma antibody (ABIN359066) 图像
特异性 This antibody reacts to PKC gamma.
纯化方法 Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
免疫原 This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.
亚型 Ig Fraction

PKC gamma目标详情

PKC gamma抗体详情 使用细节 贮存及处理 References for anti-PKC gamma antibody (ABIN359066) 图像 回页首
抗原
别名 PRKCG (PRKCG Antibody 摘要)
背景 Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).Synonyms: PKC gamma, PKC-gamma, PKCC, PKCG, Protein kinase C gamma type
基因ID 5582, 9606
UniProt P05129
途径 WNT signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling

使用细节

PKC gamma抗体详情 PKC gamma目标详情 贮存及处理 References for anti-PKC gamma antibody (ABIN359066) 图像 回页首
应用备注 ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

PKC gamma抗体详情 PKC gamma目标详情 使用细节 References for anti-PKC gamma antibody (ABIN359066) 图像 回页首
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PKC gamma antibody (ABIN359066)

PKC gamma抗体详情 PKC gamma目标详情 使用细节 贮存及处理 图像 回页首
背景

Uchino, Sakai, Kashiwagi, Shirai, Shinohara, Hirose, Iino, Yamamura, Saito: "Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 2254-61, 2004

van de Warrenburg, Verbeek, Piersma, Hennekam, Pearson, Knoers, Kremer, Sinke: "Identification of a novel SCA14 mutation in a Dutch autosomal dominant cerebellar ataxia family." in: Neurology, Vol. 61, Issue 12, pp. 1760-5, 2003

Yabe, Sasaki, Chen, Raskind, Bird, Yamashita, Tsuji, Kikuchi, Tashiro: "Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma." in: Archives of neurology, Vol. 60, Issue 12, pp. 1749-51, 2003

Ananthanarayanan, Stahelin, Digman, Cho: "Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46886-94, 2003

Correia, Duarte, Faro, Pires, Carvalho: "Protein kinase C gamma associates directly with the GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor subunit. Effect on receptor phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6307-13, 2003

图像

PKC gamma抗体详情 PKC gamma目标详情 使用细节 贮存及处理 References for anti-PKC gamma antibody (ABIN359066) 回页首
厂商提供的图像
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, gamma (PRKCG) (C-Term) antibody (ABIN359066) Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibo...
Western Blotting (WB) image for anti-Protein Kinase C, gamma (PRKCG) (C-Term) antibody (ABIN359066) Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma (...