anti-牛 CD36 抗体 for Western Blotting

Recommended CD36 Antibody (supplied by: Log in to see )

抗原
CD36 Molecule (thrombospondin Receptor) (CD36) 抗体
  • BDPLT10
  • CHDS7
  • FAT
  • GP3B
  • GP4
  • GPIV
  • PASIV
  • SCARB3
  • Fat
  • Scarb3
  • GPIIIB
  • PAS-4
  • zgc:92513
  • CD36 molecule
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Cd36
  • cd36
抗原表位
AA 100-200
16
16
15
12
11
9
6
4
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
适用
牛, 人, 小鼠, 猪, 灵长类, 大鼠
378
163
103
12
7
6
4
3
3
3
3
1
宿主

263
153
48
12
2
克隆类型
多克隆
标记
This CD36 antibody is un-conjugated
52
27
22
21
13
9
8
6
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
应用范围
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
295
164
135
101
64
47
43
40
39
29
13
3
3
3
3
3
3
2
2
2
2
1
1
1
1
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产品编号 ABIN152911
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.743937 ABIN2783229 IHC WB Rabbit N-Term Log in to see Polyclonal 1
16.743937 ABIN2783230 IHC WB Rabbit N-Term Log in to see Polyclonal 1
7 ABIN321373 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1732842 IHC WB Biotin Rabbit IgG AA 100-200 Log in to see Polyclonal 0
1 ABIN1732843 IHC WB DyLight 650 Rabbit IgG AA 100-200 Log in to see Polyclonal 0
1 ABIN1732846 IHC WB HiLyte Fluor 488 Rabbit IgG AA 100-200 Log in to see Polyclonal 0
1 ABIN1732847 IHC WB DyLight 550 Rabbit IgG AA 100-200 Log in to see Polyclonal 0
1 ABIN1732845 IHC WB HRP Rabbit IgG AA 100-200 Log in to see Polyclonal 0
1 ABIN1732844 IHC WB DyLight 488 Rabbit IgG AA 100-200 Log in to see Polyclonal 0
-8.269841 ABIN268878 IHC (fro) IHC (p) WB HRP Rabbit AA 100-200 Log in to see Polyclonal 2
-12.769841 ABIN268877 ICC IF IHC (fro) IHC (p) WB Biotin Rabbit AA 100-200 Log in to see Polyclonal 0
-12.769841 ABIN446289 ICC IF IHC (fro) IHC (p) WB DyLight 488 Rabbit AA 100-200 Log in to see Polyclonal 0

General

抗原 CD36 Molecule (thrombospondin Receptor) (CD36) 抗体
抗原表位 AA 100-200
(16), (16), (15), (12), (11), (9), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
适用 牛, 人, 小鼠, 猪, 灵长类, 大鼠
(378), (163), (103), (12), (7), (6), (4), (3), (3), (3), (3), (1)
宿主
(263), (153), (48), (12), (2)
克隆类型
多克隆
标记 This CD36 antibody is un-conjugated
(52), (27), (22), (21), (13), (9), (8), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(295), (164), (135), (101), (64), (47), (43), (40), (39), (29), (13), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 47个引用可参考
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CD36抗体详情

CD36目标详情 使用细节 贮存及处理 References for anti-CD36 antibody (ABIN152911) 图像
纯化方法 Immunogen affinity purified
免疫原 A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Plasmids, Primers & others

CD36目标详情

CD36抗体详情 使用细节 贮存及处理 References for anti-CD36 antibody (ABIN152911) 图像 回页首
抗原
别名 CD36 (CD36 Antibody 摘要)
背景 Gene Symbol: CD36
分子量 Theoretical MW: 75 kDa
基因ID 948
UniProt P16671
途径 TLR signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism

使用细节

CD36抗体详情 CD36目标详情 贮存及处理 References for anti-CD36 antibody (ABIN152911) 图像 回页首
应用备注 Western Blot 1:500-1:2000, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-FrozenThis CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Protocol specific for CD36 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBST for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk, 1 % BSA in TBST for 1 hour.
. Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 degrees Celcius.
. Wash the membrane in TBST 5 times for 5 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBST 5 times for 5 minutes each.
. Apply the detection reagent of choice in accordance with the manufacturers instructions.IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
限制 仅限研究用

贮存及处理

CD36抗体详情 CD36目标详情 使用细节 References for anti-CD36 antibody (ABIN152911) 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS
Buffer contains: 0.02 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C,-20 °C
储存方法 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD36 antibody (ABIN152911)

CD36抗体详情 CD36目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Lee, Han, Park, Baek, Song, Choi, Takuwa, Ryu, Kim, He, Schuchman, Bae, Jin: "Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease." in: Nature communications, Vol. 9, Issue 1, pp. 1479, 2018

Rozovski, Harris, Li, Liu, Jain, Ferrajoli, Burger, Thompson, Jain, Wierda, Keating, Estrov: "STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells." in: Oncotarget, Vol. 9, Issue 30, pp. 21268-21280, 2018

Misra, Feng, Chandran, Kabir, Rotllan, Aryal, Sheikh, Ding, Qin, Fernández-Hernando, Tellides, Greif: "Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells." in: Nature communications, Vol. 9, Issue 1, pp. 2073, 2018

Sankar, Donegan, Shah, Reddi, Wood: "Heme and hemoglobin suppress amyloid β-mediated inflammatory activation of mouse astrocytes." in: The Journal of biological chemistry, Vol. 293, Issue 29, pp. 11358-11373, 2018

Li, Lu, Ru, Lopes-Virella, Lyons, Huang: "Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro." in: American journal of physiology. Endocrinology and metabolism, 2018

Fang, Wang, Yao, Li, Wang, Liu, Tao, Sun, Liao: "The adhesion and migration of microglia to β-amyloid (Aβ) is decreased with aging and inhibited by Nogo/NgR pathway." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 210, 2018

Lavie, Dyugovskaya, Polyakov, Rogovoy, Leder: "Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro." in: Journal of visualized experiments : JoVE, Issue 119, 2017

Chen, Huang, Yang, Xin, Cui, Xie, Silverstein: "Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 8, pp. 1462-1469, 2017 (Sample species: Mouse (Murine)).

Xian, Ding, Dieckmann, Zhou, Plattner, Liu, Parks, Hammer, Boucher, Tsai, Herz: "LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis." in: eLife, Vol. 6, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Guri, Colombi, Dazert, Hindupur, Roszik, Moes, Jenoe, Heim, Riezman, Riezman, Hall: "mTORC2 Promotes Tumorigenesis via Lipid Synthesis." in: Cancer cell, Vol. 32, Issue 6, pp. 807-823.e12, 2017 (Sample species: Mouse (Murine)).

Souza, Bocharov, Baranova, Vishnyakova, Huang, Wilkins, Hu, Street, Alvarez-Prats, Mullick, Patterson, Remaley, Eggerman, Yuen, Star: "Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation." in: Kidney international, Vol. 89, Issue 4, pp. 809-22, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Salameh, Daquinag, Staquicini, An, Hajjar, Pasqualini, Arap, Kolonin: "Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue." in: JCI insight, Vol. 1, Issue 10, 2016

Garbacz, Lu, Miller, Poloyac, Eyre, Mayrhofer, Xu, Ren, Xie: "Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance." in: Molecular and cellular biology, Vol. 36, Issue 21, pp. 2715-2727, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 Method employed by authors: Western Blotting (WB)

Vaillant, Lauzier, Ruiz, Shi, Lachance, Rivard, Bolduc, Thorin, Tardif, Des Rosiers: "Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 4, pp. H991-H1003, 2016

Konstantynowicz-Nowicka, Harasim, Baranowski, Chabowski: "New evidence for the role of ceramide in the development of hepatic insulin resistance." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116858, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Zidek, Ackermann, Hartleben, Eichwald, Kortman, Kiehntopf, Leutz, Sonenberg, Wang, von Maltzahn, Müller, Calkhoven: "Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice." in: EMBO reports, Vol. 16, Issue 8, pp. 1022-36, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Barroso, Rodríguez-Rodríguez, Chacón, Maymó-Masip, Ferrer, Salvadó, Salmerón, Wabistch, Palomer, Vendrell, Wahli, Vázquez-Carrera: "PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation." in: Biochimica et biophysica acta, Vol. 1852, Issue 5, pp. 1049-58, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Bartels, Christoffersen, Lindholm, Nielsen: "Altered metabolism of LDL in the arterial wall precedes atherosclerosis regression." in: Circulation research, Vol. 117, Issue 11, pp. 933-42, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Zhao, Xiong, Lechner, Klenotic, Hamburg, Hulver, Khare, Oriss, Mangalmurti, Chan, Zhang, Ross, Stolz, Rosengart, Pilewski, Ray, Ray, Silverstein, Lee: "Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury." in: Mucosal immunology, Vol. 7, Issue 2, pp. 440-8, 2014

图像

CD36抗体详情 CD36目标详情 使用细节 贮存及处理 References for anti-CD36 antibody (ABIN152911) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml ABIN152911, ...
Immunohistochemistry (IHC) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Staining of humnan adipocytes using ABIN152911.
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody [ABIN152911] - CD36 antib...
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody - Mouse liver, image is ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Total protein from Human Skin and Adipose tissue...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Alcohol feeding significantly up-regulated CD36 ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Mouse primary muscle cell lysate. This image was...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Alcohol feeding significantly up-regulated CD36 ...