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|抗原||EPH Receptor A2 (EPHA2) 抗体|
|抗原表位||AA 38-67, N-Term 其他选择|
|适用||Hamster, 人, 小鼠 其他选择|
|标记||This EPH Receptor A2 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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EPH Receptor A2抗体详情EPH Receptor A2目标详情 使用细节 贮存及处理 References for anti-EPH Receptor A2 antibody (ABIN359790) 图像
|特异性||This antibody recognizes EphA2 (N-term)|
|纯化方法||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|免疫原||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~67 amino acids from the N-terminal region of Human EphA2.|
EPH Receptor A2目标详情EPH Receptor A2抗体详情 使用细节 贮存及处理 References for anti-EPH Receptor A2 antibody (ABIN359790) 图像 回页首
|别名||EPHA2 / ECK (EPHA2 Antibody 摘要)|
|背景||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: Eph receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK|
使用细节EPH Receptor A2抗体详情 EPH Receptor A2目标详情 贮存及处理 References for anti-EPH Receptor A2 antibody (ABIN359790) 图像 回页首
ELISA: 1/1,000. Western blot: 1/100-1/500. Flow Cytometry: 1/10-1/50. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理EPH Receptor A2抗体详情 EPH Receptor A2目标详情 使用细节 References for anti-EPH Receptor A2 antibody (ABIN359790) 图像 回页首
|缓冲液||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-EPH Receptor A2 antibody (ABIN359790)EPH Receptor A2抗体详情 EPH Receptor A2目标详情 使用细节 贮存及处理 图像 回页首
Lindberg, Hunter: "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6316-24, 1991
图像EPH Receptor A2抗体详情 EPH Receptor A2目标详情 使用细节 贮存及处理 References for anti-EPH Receptor A2 antibody (ABIN359790) 回页首