Fully biologically active when compared to standard. The ED50 determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of >, 2.0 × 106 IU/mg in the presence of 50pg/ml rHuIL-1alpha.
纯度
> 95 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rHuIL-1ra as determined by LAL method
IL1RN
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
4 °C
Hedl, Abraham: "Nod2-induced autocrine interleukin-1 alters signaling by ERK and p38 to differentially regulate secretion of inflammatory cytokines." in: Gastroenterology, Vol. 143, Issue 6, pp. 1530-43, (2012) (PubMed).
Interleukin-1 receptor antagonist (IL-1ra) is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra.The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF. Synonym: Interleukin-1 receptor antagonist (IL-1ra), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
分子量
Approximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.