Purified recombinant Human IFN beta protein Expression System: CHO cells Bioactivity: Assay#1: The biological activity of PeproTech's human IFN-gamma was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activitywas determined to be >5 x 10 8 units/mg, Assay#2: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/mL.
Each Investigator should determine their own optimal working dilution for specific applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in water to a concentration of 0.1-1.0 g/mL. Do not vortex. For extended storage, dilute further in a buffer containing a carrier protein (ie: 0.1 % BSA).
缓冲液
Lyophilized from 10 mM sodium citrate, with 100 mM NaCl, and 10 mM Sucrose.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNa is an acid stable molecule that signals through IFNa/betaR, which is also used by IFNbeta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNa contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Alternative Names: Leukocyte interferon antibody, RMMB1 antibody, Interferon beta antibody, Type I interferon antibody, B cell interferon antibody