FCGR1A Protein (AA 16-281) (His-Avi Tag)
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- 抗原 See all FCGR1A 蛋白
- FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 16-281
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This FCGR1A protein is labelled with His-Avi Tag.
- 应用范围
- SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)
- 原理
- Human Fc gamma RI / CD64 protein
- 序列
- QVDTTKAVIT LQPPWVSVFQ EETVTLHCEV LHLPGSSSTQ WFLNGTATQT STPSYRITSA SVNDSGEYRC QRGLSGRSDP IQLEIHRGWL LLQVSSRVFT EGEPLALRCH AWKDKLVYNV LYYRNGKAFK FFHWNSNLTI LKTNISHNGT YHCSGMGKHR YTSAGISVTV KELFPAPVLN ASVTSPLLEG NLVTLSCETK LLLQRPGLQL YFSFYMGSKT LRGRNTSSEY QILTARREDS GLYWCEAATE DGNVLKRSPE LELQVLGGGL NDIFEAQKIE WHEGGGENLY FQSGGHHHHH HHHHH
- 特异性
- IgG
- 产品特性
- The sequence of the extracellular domain of human CD64 (Gln 16-Leu 281) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
- 纯化方法
- Nickel and SEC
- 纯度
- > 95 % by SEC-HPLC
- 内毒素水平
- <1.0 EU per mg
- Biological Activity Comment
- Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RI / CD64 protein can bind to anti-HER2 human IgG1 (trastuzumab) immobilized on a Protein A chip with an affinity constant (KD) of 0.1 nM.
- Top Product
- Discover our top product FCGR1A 蛋白
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Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-281) protein (His-Avi Tag,Biotin)
FCGR1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-115) protein (GST tag)FCGR1A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 25-297) (Active) protein (His tag)FCGR1A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 11-288) (Active) protein (His tag,AVI tag,Biotin)FCGR1A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 1-374) protein (GST tag)FCGR1A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (Active) protein (His tag)FCGR1A 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-281) (Active) protein (His tag)FCGR1A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-288) (Active) protein (His tag,AVI tag,Biotin)FCGR1A 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C,-80 °C
- 储存方法
- Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
- 有效期
- 12 months
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- 抗原
- FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
- 别名
- CD64 (FCGR1A 产品)
- 别名
- CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, FcgammaRI Protein, Fcgr1 Protein, Fcgr1b Protein, AI323638 Protein, AV092959 Protein, IGGHAFC Protein, FCGR1A Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1a Protein, Fcgr1 Protein
- 背景
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CD64, CD64A, FCGR1, FCGRI, FCGR1A, FCGRIA, FCR1, FCRI, IGFR1
Background: IGFRI,High affinity immunoglobulin gamma Fc receptor I, also known as FcγRI or CD64, is a type I integral membrane glycoprotein. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes, macrophages, dendritic cells and activated granulocytes. CD64 binds with high affinity to the Fc domain of IgG and it plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). CD64 is structurally composed of three extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD64 is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD64. - 分子量
- 34.4 kDa
- UniProt
- P12314
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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