P-Selectin Protein (Fc Tag,His tag)
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- 抗原 See all P-Selectin (SELP) 蛋白
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This P-Selectin protein is labelled with Fc Tag,His tag.
- 原理
- Active Recombinant Human SELP/P-Selectin/CD62P Protein
- 序列
- WTYHYSTKAY SWNISRKYCQ NRYTDLVAIQ NKNEIDYLNK VLPYYSSYYW IGIRKNNKTW TWVGTKKALT NEAENWADNE PNNKRNNEDC VEIYIKSPSA PGKWNDEHCL KKKHALCYTA SCQDMSCSKQ GECLETIGNY TCSCYPGFYG PECEYVRECG ELELPQHVLM NCSHPLGNFS FNSQCSFHCT DGYQVNGPSK LECLASGIWT NKPPQCLAAQ CPPLKIPERG NMTCLHSAKA FQHQSSCSFS CEEGFALVGP EVVQCTASGV WTAPAPVCKA VQCQHLEAPS EGTMDCVHPL TAFAYGSSCK FECQPGYRVR GLDMLRCIDS GHWSAPLPTC EAISCEPLES PVHGSMDCSP SLRAFQYDTN CSFRCAEGFM LRGADIVRCD NLGQWTAPAP VCQALQCQDL PVPNEARVNC SHPFGAFRYQ SVCSFTCNEG LLLVGASVLQ CLATGNWNSV PPECQAIPCT PLLSPQNGTM TCVQPLGSSS YKSTCQFICD EGYSLSGPER LDCTRSGRWT DSPPMCEAIK CPELFAPEQG SLDCSDTRGE FNVGSTCHFS CDNGFKLEGP NNVECTTSGR WSATPPTCKG IASLPTPGLQ CPALTTPGQG TMYCRHHPGT FGFNTTCYFG CNAGFTLIGD STLSCRPSGQ WTAVTPACRA VKCSELHVNK PIAMNCSNLW GNFSYGSICS FHCLEGQLLN GSAQTACQEN GHWSTTVPTC QAGPLTIQEA
- 特异性
- Trp42-Ala771
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <1EU/μg
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to Human SELP coated plates (10 μg/mL with 100 μL/well), > 80% cells will adhere after 1 hour at 37°C.|2.Measured by its binding ability in a functional ELISA.Immobilized PE Mouse Anti-Human CD62P Antibody at 1μg/mL (25 μL/well) can bind Human CD62P with a linear range of 0.46-7.82 ng/mL.
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- Discover our top product SELP 蛋白
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His-Avi Tag,Biotin)
SELP 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)SELP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 1-829) protein (GST tag)SELP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (Active) protein (His tag)SELP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His tag,AVI tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)Crystallography grade SELP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) protein (His tag)Crystallography grade SELP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-830) protein (rho-1D4 tag)Crystallography grade SELP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- 别名
- SELP/P-Selectin/CD62P (SELP 产品)
- 别名
- PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein
- 背景
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Description: P selectin (SELP) is a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.
Name: CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL,CD62P/P-selectin,SELP,CD62P,GMP140,GRMP,LECAM3,PADGEM,PSEL
- 基因ID
- 6403
- UniProt
- P16109
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