P-Selectin Protein (His tag)
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- 抗原 See all P-Selectin (SELP) 蛋白
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This P-Selectin protein is labelled with His tag.
- 原理
- Recombinant Mouse SELP/selectin P/P-selectin Protein (His Tag)(Active)
- 序列
- Met1-Ala709
- 产品特性
- A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >70% cells will adhere specifically after 60 minutes at 37°C.
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His-Avi Tag,Biotin)
SELP 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)SELP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 1-829) protein (GST tag)SELP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His tag,AVI tag)SELP 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)Crystallography grade SELP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) protein (His tag)Crystallography grade SELP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-830) protein (rho-1D4 tag)Crystallography grade SELP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-768) protein (rho-1D4 tag)Crystallography grade SELP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- 别名
- SELP/selectin P/P-selectin (SELP 产品)
- 别名
- PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein
- 背景
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Background: P selectin (SELP) is a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
Synonym: CD62P,GMP-140,Grmp,LECAM3,PADGEM
- 分子量
- 74 kDa
- UniProt
- Q01102
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