RSPO1 Protein (His tag)
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- 抗原 See all RSPO1 蛋白
- RSPO1 (R-Spondin 1 (RSPO1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This RSPO1 protein is labelled with His tag.
- 原理
- Active Recombinant Human R-spondin-1 Protein
- 序列
- RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
- 特异性
- Arg31-Ala263
- 纯度
- > 95 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- 1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.|2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)|3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1.
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- Discover our top product RSPO1 蛋白
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R-Spondin 1 (RSPO1) protein (His tag)
RSPO1 宿主: 人 宿主: CHO Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)RSPO1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- 抗原
- RSPO1 (R-Spondin 1 (RSPO1))
- 别名
- R-spondin-1 (RSPO1 产品)
- 别名
- RGD1565558 Protein, CRISTIN3 Protein, RSPO Protein, R-spondin Protein, Rspondin Protein, wu:fj81e05 Protein, zgc:92153 Protein, R-spondin 1 Protein, Rspo1 Protein, RSPO1 Protein, LOC100049655 Protein, rspo1 Protein
- 背景
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Description: This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.
Name: RSPO1,CRISTIN3,RSPO
- 基因ID
- 284654
- UniProt
- Q2MKA7
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