RSPO1 Protein (AA 21-146) (His tag)
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- 抗原 See all RSPO1 蛋白
- RSPO1 (R-Spondin 1 (RSPO1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 21-146
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This RSPO1 protein is labelled with His tag.
- 序列
- AA 21-146
- 产品特性
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.7 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- 纯度
- >95 % as determined by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product RSPO1 蛋白
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R-Spondin 1 (RSPO1) protein (His tag)
RSPO1 宿主: 人 宿主: CHO Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)RSPO1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)Crystallography grade RSPO1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)Crystallography grade RSPO1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
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- 抗原
- RSPO1 (R-Spondin 1 (RSPO1))
- 别名
- R-Spondin 1 (RSPO1 产品)
- 别名
- RGD1565558 Protein, CRISTIN3 Protein, RSPO Protein, R-spondin Protein, Rspondin Protein, wu:fj81e05 Protein, zgc:92153 Protein, R-spondin 1 Protein, Rspo1 Protein, RSPO1 Protein, LOC100049655 Protein, rspo1 Protein
- 背景
- R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. R-Spondin-1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. R-Spondin-1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK1 for binding to the Wnt co receptors, Kremen and LRP6, reducing their DKK1 mediated internalization. Reports differ on whether R-spondin 1 binds LRP6 directly.
- 分子量
- 14.6 kDa
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