Prothrombin Protein (His tag)
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- 抗原 See all Prothrombin (F2) 蛋白
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Prothrombin protein is labelled with His tag.
- 原理
- Recombinant human Prothrombin/F2 Protein
- 序列
- QHVFLAPQQA RSLLQRVRRA NTFLEEVRKG NLERECVEET CSYEEAFEAL ESSTATDVFW AKYTACETAR TPRDKLAACL EGNCAEGLGT NYRGHVNITR SGIECQLWRS RYPHKPEINS TTHPGADLQE NFCRNPDSST TGPWCYTTDP TVRRQECSIP VCGQDQVTVA MTPRSEGSSV NLSPPLEQCV PDRGQQYQGR LAVTTHGLPC LAWASAQAKA LSKHQDFNSA VQLVENFCRN PDGDEEGVWC YVAGKPGDFG YCDLNYCEEA VEEETGDGLD EDSDRAIEGR TATSEYQTFF NPRTFGSGEA DCGLRPLFEK KSLEDKTERE LLESYIDGRI VEGSDAEIGM SPWQVMLFRK SPQELLCGAS LISDRWVLTA AHCLLYPPWD KNFTENDLLV RIGKHSRTRY ERNIEKISML EKIYIHPRYN WRENLDRDIA LMKLKKPVAF SDYIHPVCLP DRETAASLLQ AGYKGRVTGW GNLKETWTAN VGKGQPSVLQ VVNLPIVERP VCKDSTRIRI TDNMFCAGYK PDEGKRGDAC EGDSGGPFVM KSPFNNRWYQ MGIVSWGEGC DRDGKYGFYT HVFRLKKWIQ KVIDQFGE
- 特异性
- Gln25-Glu622
- 纯度
- > 97 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1EU/μg
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- Discover our top product F2 蛋白
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Coagulation Factor II (thrombin) (F2) (AA 44-622) protein (His tag)Crystallography grade F2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 44-618) protein (His tag)Crystallography grade F2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 浓度
- 0.73 mg/mL
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 别名
- Prothrombin/F2 (F2 产品)
- 别名
- PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, zgc:66319 Protein, wu:fb57c10 Protein, wu:fd42e09 Protein, wu:fd59d01 Protein, prothrombin Protein, F2 Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, coagulation factor II (thrombin) Protein, F2 Protein, f2 Protein
- 背景
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Description: Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions, factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin.
Name: PT, THPH1, RPRGL2
- 基因ID
- 2147
- UniProt
- P00734
- 途径
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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