Prothrombin Protein (His tag)
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- 抗原 See all Prothrombin (F2) 蛋白
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Prothrombin protein is labelled with His tag.
- 原理
- Recombinant Mouse Coagulation Factor II/FII/F2 Protein (His Tag)(Active)
- 序列
- Met 1-Gly 618
- 产品特性
- A DNA sequence encoding the full length of mouse F2 (NP_034298.1) (Met 1-Gly 618) was expressed, with a C-terminal polyhistidine tag.
- 纯度
- > 96 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC R&D Systems, Catalog # ES011.The specific activity is > 2000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
- Top Product
- Discover our top product F2 蛋白
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Coagulation Factor II (thrombin) (F2) (AA 1-622) protein (GST tag)
F2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Coagulation Factor II (thrombin) (F2) (AA 263-362) protein (GST tag)F2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Coagulation Factor II (thrombin) (F2) (AA 364-622) protein (His tag)Crystallography grade F2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 361-618) protein (His tag)Crystallography grade F2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 44-622) protein (His tag)Crystallography grade F2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Coagulation Factor II (thrombin) (F2) (AA 44-618) protein (His tag)Crystallography grade F2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 说明
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85 kDa
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 别名
- Coagulation Factor II (F2 产品)
- 别名
- PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, zgc:66319 Protein, wu:fb57c10 Protein, wu:fd42e09 Protein, wu:fd59d01 Protein, prothrombin Protein, F2 Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, coagulation factor II (thrombin) Protein, F2 Protein, f2 Protein
- 背景
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Background: Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions, factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Synonym: Cf-2,Cf2,FII
- 分子量
- 69.3 kDa
- NCBI登录号
- NP_034298
- 途径
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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