Cathepsin B Protein (CTSB) (His tag)
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- 抗原 See all Cathepsin B (CTSB) 蛋白
- Cathepsin B (CTSB)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Cathepsin B protein is labelled with His tag.
- 原理
- Recombinant Human Cathepsin B Protein
- 序列
- RSRPSFHPLS DELVNYVNKR NTTWQAGHNF YNVDMSYLKR LCGTFLGGPK PPQRVMFTED LKLPASFDAR EQWPQCPTIK EIRDQGSCGS CWAFGAVEAI SDRICIHTNA HVSVEVSAED LLTCCGSMCG DGCNGGYPAE AWNFWTRKGL VSGGLYESHV GCRPYSIPPC EHHVNGSRPP CTGEGDTPKC SKICEPGYSP TYKQDKHYGY NSYSVSNSEK DIMAEIYKNG PVEGAFSVYS DFLLYKSGVY QHVTGEMMGG HAIRILGWGV ENGTPYWLVA NSWNTDWGDN GFFKILRGQD HCGIESEVVA GIPRTDQYWE KI
- 特异性
- Arg18-Ile339
- 纯度
- > 97 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- <1EU/μg
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC . The specific activity is >2,500 pmoles/min/μg.
- Top Product
- Discover our top product CTSB 蛋白
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Cathepsin B (CTSB) (AA 1-339) protein (GST tag)
CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (AA 224-333) protein (GST tag)CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (AA 21-339) protein (GST tag)CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) protein (His tag)CTSB 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Cathepsin B (CTSB) (Active) protein (His tag)CTSB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Cathepsin B (CTSB) protein (His tag)CTSB 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Cathepsin B (CTSB) (AA 80-126) protein (His tag)Crystallography grade CTSB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cathepsin B (CTSB) (AA 80-126) protein (His tag)Crystallography grade CTSB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cathepsin B (CTSB) (AA 129-333) protein (His tag)Crystallography grade CTSB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cathepsin B (CTSB) (AA 80-333) protein (His tag)Crystallography grade CTSB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- 浓度
- 0.62 mg/mL
- 缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- 抗原
- Cathepsin B (CTSB)
- 别名
- Cathepsin B (CTSB 产品)
- 别名
- APPS Protein, CPSB Protein, CB Protein, apps Protein, cpsb Protein, LOC692390 Protein, CTSB Protein, cathepsin B Protein, cathepsin B S homeolog Protein, CTSB Protein, Ctsb Protein, ctsb.S Protein, LOC692390 Protein
- 背景
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Description: Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
Name: Cathepsin B, 3.4.22.1, APP secretase, APPS, Cathepsin B1,CTSB
- 基因ID
- 1508
- UniProt
- P07858
- 途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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