CRISPR-Cas9 protein (NLS)
宿主: Streptococcus pyogenes
宿主: 大肠杆菌（E. Coli）
Recombinant
GEEN
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- 抗原
- CRISPR-Cas9
- 蛋白类型
- Recombinant
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宿主
- Streptococcus pyogenes
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资源
- 大肠杆菌（E. Coli）
- 标记
- NLS
- 应用范围
- Genome Editing with Engineered Nucleases (GEEN)
- 原理
- Ultra NLS-Cas9-basic
- 产品特性
- Recombinant Streptococcus pyogenes Cas9 gene expressed in an Escherichia coli with a N-terminal nucleic localization signal (NLS).
- 纯化方法
- Escherichia coli expression system
- 内毒素水平
- 10 EU/mg as analyzed by gel clotting method
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- 应用备注
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CRISPR Genomic editing
The optimal working dilution should be determined by the end user. - 实验流程
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Cleavage assay: A 20 uL reaction in 1xCas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng Ultra NLS-Cas9-basic for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- In 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH 8.0 (50 % glycerol).
- 储存液
- Other preservative
- 储存条件
- -20 °C
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- 抗原
- CRISPR-Cas9
- 别名
- Cas9 nuclease
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