CRISPR-Cas9 (Active) 蛋白
宿主: Streptococcus pyogenes
宿主: 大肠杆菌（E. Coli）
Recombinant
GEEN, EP
Active
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- 抗原
- CRISPR-Cas9
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- Streptococcus pyogenes
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)
- 原理
- Cas9 nuclease
- 产品特性
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Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli .
- 纯化方法
- E. coli expression system
- Biological Activity Comment
- 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.
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- 应用备注
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CRISPR Genome editing
The optimal working dilution should be determined by the end user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 % Glycerol PH 7.4
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- CRISPR-Cas9
- 别名
- Cas9 nuclease
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