Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)
原理
Cas9-C-NLS nuclease
产品特性
Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with a C-terminal nucleic localization signal (NLS).
纯化方法
E. coli expression system
内毒素水平
< 0.1 eu/μg
Biological Activity Comment
20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.