VEGF121 Protein (His-Avi Tag,Biotin)
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- 抗原 See all VEGF121 蛋白
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This VEGF121 protein is labelled with His-Avi Tag,Biotin.
- 序列
- Ala27-Arg147
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human VEGF R1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 36.5ng/ml determined by ELISA. See testing image for detail.
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Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) protein (His-Avi Tag)
VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) proteinVEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 28-147) (Active) protein (His tag)VEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (Isoform 121) proteinVEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (Isoform 121) protein (His tag)VEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- 别名
- VEGF121 (VEGF121 产品)
- 背景
- VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
- 分子量
- 17 kDa. Due to glycosylation, the protein migrates to 18 kDa/22-25 kDa based on Tris-Bis PAGE result.
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