VEGF121 Protein (AA 28-147) (His tag)
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- 抗原 See all VEGF121 蛋白
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- 生物活性
- Active
- 产品特性
- AA 28-147
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This VEGF121 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGF121. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. The result was shown in Figure 2. It was obvious that VEGF121 significantly increased cell viability of ECV-304 cells.
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- Discover our top product VEGF121 蛋白
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Vascular Endothelial Cell Growth Factor 121 (VEGF121) protein (His-Avi Tag,Biotin)
VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) protein (His-Avi Tag)VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) proteinVEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (Isoform 121) proteinVEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (Isoform 121) protein (His tag)VEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS PAGE SDS
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- 应用备注
- Isoelectric Point: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
- 别名
- Vascular Endothelial Growth Factor 121 (VEGF121) (VEGF121 产品)
- 分子量
- 17/18kDa
- UniProt
- P15692
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