TRAIL (TNFSF10)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
蛋白类型
Recombinant
产品特性
Trimer
宿主
人
资源
HEK-293 Cells
标记
This TRAIL protein is labelled with His-DYKDDDDK Tag.
序列
Gly118-Gly281
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized TRAIL Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
TNFSF10
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
有效期
12 months
抗原
TRAIL (TNFSF10)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
Apo-2L, TRAIL, CD253, TNFSF10, Apo-2 ligand, APO2L, TL2,Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.
分子量
60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.