TNFRSF12A
(Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
蛋白类型
Recombinant
产品特性
AA 28-80
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF12A protein is labelled with Fc Tag.
原理
Human TNFRSF12A/TWEAKR Protein
序列
Glu28-Per80
产品特性
Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Glu28-Per80.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNFRSF12A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA. See testing image for detail.
TNFRSF12A
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TNFRSF12A
宿主: 小鼠
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF12A
(Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
Thyroid cancer (TC) is the most well-known endocrine neoplasia as well as a common malignant tumor in the head and neck.TNFRSF12A expression may be a potential useful prognostic molecular biomarker of bad survival in thyroid cancer, in addition, PPAR signaling pathway, insulin signaling pathway, mTOR signaling pathway may be the key pathway controlled by TNFRSF12A in thyroid cancer. Further experimental ought to be performed to demonstrate the biologic effect of TNFRSF12A.
分子量
32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.