SIRPA Protein (Fc-Avi Tag,Biotin)
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- 抗原 See all SIRPA 蛋白
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This SIRPA protein is labelled with Fc-Avi Tag,Biotin.
- 序列
- Glu31-Arg370
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD47, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
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Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)
SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-His Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) protein (His tag)SIRPA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (Biotin,His-Avi Tag)SIRPA 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) (Active) protein (Fc Tag)SIRPA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- 别名
- SIRP alpha (SIRPA 产品)
- 别名
- BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein
- 背景
- CD172a, BIT, MFR, MYD1, MYD-1, P84, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, SIRP alpha, SIRPA, Sirp-alpha-1, SIRPalpha2, Sirp-alpha-2, Sirp-alpha-3,Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
- 分子量
- 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.
- UniProt
- P78324
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