SIRPA Protein (Biotin,His-Avi Tag)
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- 抗原 See all SIRPA 蛋白
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 人细胞
- 标记
- This SIRPA protein is labelled with Biotin,His-Avi Tag.
- 原理
- Recombinant Human Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His-Avi) Biotinylated
- 序列
- Glu31-Arg370
- 产品特性
- Biotinylated Recombinant Human Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His, Avi tag at the C-terminus.
- 纯度
- >95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Immobilized Human SIRPA-His-Avi(Cat#CY28) at 10μg/ml (100 μl/well) can bind Human CD47-Fc(Cat#CG18). The ED50 of Human CD47-Fc(Cat#CG18) is 898 ng/ml.
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- Discover our top product SIRPA 蛋白
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Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)
SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-His Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Signal-Regulatory Protein alpha (SIRPA) protein (Fc-Avi Tag,Biotin)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) protein (His tag)SIRPA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) (Active) protein (Fc Tag)SIRPA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 说明
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50-80 kDa
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
- 别名
- CD172a (SIRPA 产品)
- 别名
- BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein
- 背景
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Background: Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal, it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Synonym: Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, SHP Substrate 1, SHPS-1, Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs, Bit, CD172 Antigen-Like Family Member A, Inhibitory Feceptor SHPS-1, Macrophage Fusion Receptor, MyD-1 Antigen, Signal-Regulatory Protein Alpha-1, Sirp-Alpha-1, Signal-Regulatory Protein Alpha-2, Sirp-Alpha-2, Signal-Regulatory Protein Alpha-3, Sirp-Alpha-3, p84, CD172a, SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP
- 分子量
- 39.9 kDa
- UniProt
- P78324
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