TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
蛋白类型
Recombinant
产品特性
Trimer
宿主
人
资源
HEK-293 Cells
标记
This TNFSF4 protein is labelled with His-DYKDDDDK Tag.
原理
Human OX40 Ligand/TNFSF4 Trimer Protein
序列
Gln51-Leu183
产品特性
Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.
纯度
> 90 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
Crystallography grade
TNFSF4
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
分子量
49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Tris-Bis PAGE result.