c-MET Protein (AA 25-932) (His-Avi Tag)
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- 抗原 See all c-MET (MET) 蛋白
- c-MET (MET) (Met Proto-Oncogene (MET))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-932
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This c-MET protein is labelled with His-Avi Tag.
- 原理
- Human HGF R/c-MET Protein
- 序列
- Glu25-Thr932
- 产品特性
- Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu25-Thr932.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
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Met Proto-Oncogene (MET) (AA 25-932) protein (Fc Tag)
MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Met Proto-Oncogene (MET) (AA 25-930) protein (His tag)MET 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Met Proto-Oncogene (MET) (AA 25-932) protein (His-Avi Tag,Biotin)MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Met Proto-Oncogene (MET) (Tyr1235Asp-Mutant) (Active) protein (GST tag)MET 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (AA 956-1390) (Active) protein (GST tag)MET 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Tyr1230Cys-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Phe1200Ile-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (AA 956-1390) (Active) protein (His-GST)MET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Tyr1230Ala-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Asp1228His-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- c-MET (MET) (Met Proto-Oncogene (MET))
- 别名
- HGF R (MET 产品)
- 别名
- AUTS9 Protein, HGFR Protein, RCCP2 Protein, c-Met Protein, AI838057 Protein, HGF Protein, Par4 Protein, Hgfr Protein, c-met Protein, MET Protein, C-MET Protein, met Protein, met-A Protein, MET proto-oncogene, receptor tyrosine kinase Protein, met proto-oncogene Protein, MET proto-oncogene, receptor tyrosine kinase L homeolog Protein, MET Protein, Met Protein, met.L Protein
- 背景
- C-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
- 分子量
- 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on Tris-Bis PAGE result.
- 途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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