c-MET Protein (AA 25-932) (Fc Tag)
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- 抗原 See all c-MET (MET) 蛋白
- c-MET (MET) (Met Proto-Oncogene (MET))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-932
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This c-MET protein is labelled with Fc Tag.
- 原理
- Human HGF R/c-MET Protein
- 序列
- Glu25-Thr932
- 产品特性
- Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu25-Thr932.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human HGF, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 46.7ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product MET 蛋白
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Met Proto-Oncogene (MET) (AA 25-932) protein (His-Avi Tag)
MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Met Proto-Oncogene (MET) (AA 25-930) protein (His tag)MET 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Met Proto-Oncogene (MET) (AA 25-932) protein (His-Avi Tag,Biotin)MET 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Met Proto-Oncogene (MET) (Tyr1235Asp-Mutant) (Active) protein (GST tag)MET 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (AA 956-1390) (Active) protein (GST tag)MET 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Tyr1230Cys-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Phe1200Ile-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (AA 956-1390) (Active) protein (His-GST)MET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Tyr1230Ala-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Met Proto-Oncogene (MET) (Asp1228His-Mutant) (Active) proteinMET 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- c-MET (MET) (Met Proto-Oncogene (MET))
- 别名
- HGF R (MET 产品)
- 别名
- AUTS9 Protein, HGFR Protein, RCCP2 Protein, c-Met Protein, AI838057 Protein, HGF Protein, Par4 Protein, Hgfr Protein, c-met Protein, MET Protein, C-MET Protein, met Protein, met-A Protein, MET proto-oncogene, receptor tyrosine kinase Protein, met proto-oncogene Protein, MET proto-oncogene, receptor tyrosine kinase L homeolog Protein, MET Protein, Met Protein, met.L Protein
- 背景
- C-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
- 分子量
- 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Tris-Bis PAGE result.
- 途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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