This FOLR1 protein is labelled with His-Avi Tag,Biotin.
序列
Arg25-Met233
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human FOLR1, His at 0.2μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
FOLR1
宿主: 大鼠
宿主: HEK-293 Cells
Recombinant
> 85 % as determined by SDS-PAGE
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
FOLR, FOLR1, FBP, Folbp1, KB cells FBP, MOv18,Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .
分子量
27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.