DKK1 Protein (AA 178-266) (Fc-Avi Tag)
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- 抗原 See all DKK1 蛋白
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 178-266
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DKK1 protein is labelled with Fc-Avi Tag.
- 原理
- Human DKK1 C terminal Domain Protein
- 序列
- Met178-His266
- 产品特性
- Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFC Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product DKK1 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc,150 mM NaCl ( pH 5.0).
- 缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM NaAc,150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 别名
- DKK1 (DKK1 产品)
- 别名
- DKK-1 Protein, SK Protein, AI414495 Protein, SGY Protein, SGY-1 Protein, SGY1 Protein, Soggy Protein, mSgy Protein, dkk1 Protein, dkk Protein, dkk-1 Protein, xdkk1 Protein, Xdkk-1 Protein, Dickkopf1 Protein, dickkopf-1 Protein, mdkk-1 Protein, dkk1-A Protein, dickkopf WNT signaling pathway inhibitor 1 Protein, dickkopf like acrosomal protein 1 Protein, dickkopf-related protein 1 Protein, dickkopf-like 1 Protein, dickkopf WNT signaling pathway inhibitor 1b Protein, dickkopf protein Protein, dickkopf WNT signaling pathway inhibitor 1 S homeolog Protein, DKK1 Protein, Dkk1 Protein, DKKL1 Protein, LOC100228789 Protein, Dkkl1 Protein, dkk1b Protein, dkk1 Protein, dickkopf Protein, dkk1.S Protein
- 背景
- Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
- 分子量
- 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
- UniProt
- O94907
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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