DKK1 Protein (AA 32-266) (His tag)
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- 抗原 See all DKK1 蛋白
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 32-266
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宿主
- Cynomolgus, 猕猴
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资源
- HEK-293 Cells
- 标记
- This DKK1 protein is labelled with His tag.
- 原理
- Cynomolgus/Rhesus macaque DKK1 Protein
- 序列
- Thr32-His266
- 产品特性
- Recombinant Cynomolgus/Rhesus macaque DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr32-His266.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus/Rhesus macaque DKK1, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product DKK1 蛋白
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Dickkopf Homolog 1 (DKK1) (AA 32-266) protein (His-Avi Tag,Biotin)
DKK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Dickkopf Homolog 1 (DKK1) (AA 32-142) protein (Fc-Avi Tag)DKK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Dickkopf Homolog 1 (DKK1) (C-Term) protein (Fc-Avi Tag,Biotin)DKK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Dickkopf Homolog 1 (DKK1) (AA 178-266) protein (Fc-Avi Tag)DKK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Dickkopf Homolog 1 (DKK1) (N-Term) protein (Fc-Avi Tag,Biotin)DKK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Dickkopf Homolog 1 (DKK1) (AA 1-272) protein (His tag)DKK1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Dickkopf Homolog 1 (DKK1) (AA 1-266) protein (GST tag)DKK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dickkopf Homolog 1 (DKK1) (AA 81-180) protein (GST tag)DKK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dickkopf Homolog 1 (DKK1) (AA 1-242) protein (GST tag)DKK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dickkopf Homolog 1 (DKK1) (AA 31-242) protein (His tag)Crystallography grade DKK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 别名
- DKK1 (DKK1 产品)
- 别名
- DKK-1 Protein, SK Protein, AI414495 Protein, SGY Protein, SGY-1 Protein, SGY1 Protein, Soggy Protein, mSgy Protein, dkk1 Protein, dkk Protein, dkk-1 Protein, xdkk1 Protein, Xdkk-1 Protein, Dickkopf1 Protein, dickkopf-1 Protein, mdkk-1 Protein, dkk1-A Protein, dickkopf WNT signaling pathway inhibitor 1 Protein, dickkopf like acrosomal protein 1 Protein, dickkopf-related protein 1 Protein, dickkopf-like 1 Protein, dickkopf WNT signaling pathway inhibitor 1b Protein, dickkopf protein Protein, dickkopf WNT signaling pathway inhibitor 1 S homeolog Protein, DKK1 Protein, Dkk1 Protein, DKKL1 Protein, LOC100228789 Protein, Dkkl1 Protein, dkk1b Protein, dkk1 Protein, dickkopf Protein, dkk1.S Protein
- 背景
- Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
- 分子量
- 26.85 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- NCBI登录号
- XP_005565907, NP_001247454
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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