Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DDR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
DDR1
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways, however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.
分子量
45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.