CD8 alpha Protein (AA 22-182) (His-Avi Tag,Biotin)
-
- 抗原 See all CD8 alpha (CD8A) 蛋白
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-182
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This CD8 alpha protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human CD8 alpha/CD8A Protein
- 序列
- Ser22-Asp182
- 产品特性
- Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Asp182.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-CD8 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CD8A 蛋白
-
CD8a Molecule (CD8A) (AA 28-196) protein (His tag)
CD8A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD8a Molecule (CD8A) (AA 22-182) (Active) protein (His tag)CD8A 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD8a Molecule (CD8A) (AA 22-182) (Active) protein (His tag,AVI tag,Biotin)CD8A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD8a Molecule (CD8A) (AA 22-182) protein (His-IF2DI Tag)CD8A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
CD8a Molecule (CD8A) (AA 22-131) protein (GST tag)CD8A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD8a Molecule (CD8A) (AA 1-235) protein (GST tag)CD8A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD8a Molecule (CD8A) (Active) protein (His tag)CD8A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
CD8a Molecule (CD8A) protein (Fc Tag)CD8A 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- 别名
- CD8 alpha (CD8A 产品)
- 别名
- cd8 Protein, LOC100136450 Protein, BB154331 Protein, Ly-2 Protein, Ly-35 Protein, Ly-B Protein, Lyt-2 Protein, zgc:136643 Protein, CD8 Protein, Leu2 Protein, MAL Protein, p32 Protein, RHACD8-4 Protein, CD8a molecule Protein, uncharacterized LOC100125537 Protein, CD8 alpha Protein, CD8 antigen, alpha chain Protein, T-cell surface glycoprotein CD8 alpha chain Protein, Cd8a Protein, LOC100125537 Protein, LOC100136450 Protein, CD8A Protein, cd8a Protein, LOC100126556 Protein
- 背景
- CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
- 分子量
- 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
- 途径
- TCR Signaling
-
