CD28 Protein (CD28) (AA 19-152) (His-Avi Tag)
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- 抗原 See all CD28 蛋白
- CD28
- 蛋白类型
- Recombinant
- 产品特性
- AA 19-152
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宿主
- 人, Cynomolgus, 猕猴
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资源
- HEK-293 Cells
- 标记
- This CD28 protein is labelled with His-Avi Tag.
- 原理
- Human/Cynomolgus/Rhesus macaque CD28 Protein
- 序列
- Asn19-Pro152
- 产品特性
- Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product CD28 蛋白
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CD28 (CD28) protein (mFc-His Tag)
CD28 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
CD28 (CD28) protein (His-Avi Tag,Biotin)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD28 (CD28) (AA 19-152) protein (Fc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD28 (CD28) (AA 19-152) protein (mFc Tag)CD28 宿主: 人, Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD28 (CD28) (AA 18-153) protein (mFc Tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (AA 18-153) protein (His tag)CD28 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28 (CD28) (Active) protein (His tag)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD28 (CD28) (Active) protein (Biotin)CD28 宿主: 人, Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 别名
- Tp44 Protein, CD28RNA Protein, cd28 Protein, CD28 molecule Protein, CD28 antigen Protein, Cd28 molecule Protein, CD28 molecule L homeolog Protein, CD28 Protein, Cd28 Protein, cd28.L Protein
- 背景
- CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
- 分子量
- 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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